Insertion junction: CLIP2.003193_1

Overview

Insertion cassette:CIB2
Side of cassette:5'
Strand:-
Strain:CLIP2.003193
Chromosome:chromosome 10
Location:2054994
Confidence (%):80
Locus systematic id Locus common name Defline Feature
Cre10.g432600 ARS18 Arylsulfatase; (1 of 19) 3.1.6.1 - Arylsulfatase / Sulfatase intron

Insertion site details

Expected flanking sequence (starting at cassette; orientation from cassette outwards): CTTCAATCCCTCCCCCCATTGCTTAATCATGAATGTACTCCCCCCCCCCG
Internal bar code:TTCAGCGATTAAAGTGGTCCGT

Confirmation - LEAP-Seq

LEAP-Seq distance:1236
LEAP-Seq percent confirming:100.0
LEAP-Seq n confirming:29
LEAP-Seq n nonconfirming:0
LEAP-Seq n unique pos:29

Suggested primers for confirmation by PCR

Suggested primer 1:AATGTCGTACAGTTGGCGGT
Suggested primer 2:TTCCTTCGCCAGCACAATCT