| Insertion cassette: | CIB2 |
| Side of cassette: | 3' |
| Strand: | - |
| Strain: | CLIP2.003201 |
| Chromosome: | chromosome 10 |
| Location: | 654917 |
| Confidence (%): | 80 |
| Locus systematic id | Locus common name | Defline | Feature |
|---|---|---|---|
| Cre10.g422000 | TMG6,TRM1 | (1 of 1) PTHR10631:SF3 - TRNA (GUANINE(26)-N(2))-DIMETHYLTRANSFERASE; tRNA (guanine-N2)-methyltransferase | CDS |
Insertion site details | |
| Expected flanking sequence (starting at cassette; orientation from cassette outwards): | CTGCCCCTCCTGCAGCACCTTGTACCCCTCCGGGATGGCGGTGGGGTCAG |
| Internal bar code: | AACCACTTAGTTTTATTTTCTT |
Confirmation - LEAP-Seq | |
| LEAP-Seq distance: | 2830 |
| LEAP-Seq percent confirming: | 100.0 |
| LEAP-Seq n confirming: | 30 |
| LEAP-Seq n nonconfirming: | 0 |
| LEAP-Seq n unique pos: | 30 |
Suggested primers for confirmation by PCR | |
| Suggested primer 1: | CGGTGATATGTGGCAAAGCG |
| Suggested primer 2: | CATACCGGATGGACCTCAGC |