Insertion junction: CLIP2.003210_1

Overview

Insertion cassette:CIB2
Side of cassette:5' truncated?
Strand:+
Strain:CLIP2.003210
Chromosome:chromosome 3
Location:237692
Confidence (%):80
Locus systematic id Locus common name Defline Feature
Cre03.g144284 CGL110 MATH-domain protein; (1 of 2) K10523 - speckle-type POZ protein (SPOP) intron

Insertion site details

Expected flanking sequence (starting at cassette; orientation from cassette outwards): AGTATGTCTCATCATCTTTGGCTGAGCTCCTGTCTCTATGGCCCGCGCAC
Internal bar code:TTGAGGACTCGACAGCATTACT

Confirmation - LEAP-Seq

LEAP-Seq distance:1359
LEAP-Seq percent confirming:90.9091
LEAP-Seq n confirming:10
LEAP-Seq n nonconfirming:1
LEAP-Seq n unique pos:11

Suggested primers for confirmation by PCR

Suggested primer 1:CCCCTACATGTGCGTTCACT
Suggested primer 2:AACAGTGGCTGAAGAGGTCG