Insertion junction: CLIP2.003213_2

Overview

Insertion cassette:CIB2
Side of cassette:5' truncated?
Strand:+
Strain:CLIP2.003213
Chromosome:chromosome 12
Location:6935817
Confidence (%):80
Locus systematic id Locus common name Defline Feature
Cre12.g561550 CDS1 Mitochondrial half-size ABC transporter, membrane protein; (1 of 2) K05661 - ATP-binding cassette, subfamily B (MDR/TAP), member 6 (ABCB6) intron

Insertion site details

Expected flanking sequence (starting at cassette; orientation from cassette outwards): TGGTGGGCCTCTGGATCATATGGTTACATGCCACACCCGATGCTGGGACC
Internal bar code:TGGTGTTCACTAAATGTACCGG

Confirmation - LEAP-Seq

LEAP-Seq distance:1406
LEAP-Seq percent confirming:100.0
LEAP-Seq n confirming:18
LEAP-Seq n nonconfirming:0
LEAP-Seq n unique pos:18

Suggested primers for confirmation by PCR

Suggested primer 1:CACATGCCCACCTGGATCTT
Suggested primer 2:ACGGTACCGAACACGACTTC