Insertion junction: CLIP2.003228_1

Overview

Insertion cassette:CIB2
Side of cassette:3' truncated?
Strand:+
Strain:CLIP2.003228
Chromosome:chromosome 1
Location:2408728
Confidence (%):80
Locus systematic id Locus common name Defline Feature
Cre01.g013801 SXD1,TCY1 (1 of 1) 5.5.1.24 - Tocopherol cyclase; Tocopherol cyclase 1 intron

Insertion site details

Expected flanking sequence (starting at cassette; orientation from cassette outwards): GACCTTGCTTCGACGCCGGCACGGTTGTCGCATATAAATCTCATAAACGT
Internal bar code:TCCGGGACAGGCAAGGCGTAGC

Confirmation - LEAP-Seq

LEAP-Seq distance:1922
LEAP-Seq percent confirming:100.0
LEAP-Seq n confirming:1
LEAP-Seq n nonconfirming:0
LEAP-Seq n unique pos:1

Suggested primers for confirmation by PCR

Suggested primer 1:TACCCAAATCCACTCGCACC
Suggested primer 2:GTGTACAGACTTGGTGGCGA