Insertion junction: CLIP2.003232_2

Overview

Insertion cassette:CIB2
Side of cassette:5'
Strand:+
Strain:CLIP2.003232
Chromosome:chromosome 1
Location:5621974
Confidence (%):80
Locus systematic id Locus common name Defline Feature
Cre01.g040150 (1 of 5) K08867 - WNK lysine deficient protein kinase [EC:2.7.11.1] (WNK, PRKWNK) intron

Insertion site details

Expected flanking sequence (starting at cassette; orientation from cassette outwards): GCGCTAGCACCGGCCGGCAGCTGGGTGCCAGTTGCAACGGGCAGGGATGC
Internal bar code:GTCAATTTCGGTTGAGGACTCG

Confirmation - LEAP-Seq

LEAP-Seq distance:6038
LEAP-Seq percent confirming:100.0
LEAP-Seq n confirming:140
LEAP-Seq n nonconfirming:0
LEAP-Seq n unique pos:140

Suggested primers for confirmation by PCR

Suggested primer 1:CATCATCCATCGCGACCTCA
Suggested primer 2:AGTGTTATTGCCGCTCACGA