Insertion junction: CLIP2.003235_1

Overview

Insertion cassette:CIB2
Side of cassette:5'
Strand:-
Strain:CLIP2.003235
Chromosome:chromosome 6
Location:3953515
Confidence (%):80
Locus systematic id Locus common name Defline Feature
Cre06.g278199 (1 of 4) 1.13.11.75 - All-trans-8'-apo-beta-carotenal 15,15'-oxygenase / 8'-apo-beta-carotenal 15,15'-oxygenase intron

Insertion site details

Expected flanking sequence (starting at cassette; orientation from cassette outwards): GTCCCCCCCCCTCCAACCCGCCCACCCCCCAGGTGACGGAGGGCCGTGTG
Internal bar code:TATAGGACTGTTAGTGGGTGTG

Confirmation - LEAP-Seq

LEAP-Seq distance:351
LEAP-Seq percent confirming:100.0
LEAP-Seq n confirming:2
LEAP-Seq n nonconfirming:0
LEAP-Seq n unique pos:2

Suggested primers for confirmation by PCR

Suggested primer 1:GAGGAGGAGGATGGGGAAGT
Suggested primer 2:CAAGTACCGCGCATTCATGG