Insertion junction: CLIP2.003239_2

Overview

Insertion cassette:CIB2
Side of cassette:5' truncated?
Strand:+
Strain:CLIP2.003239
Chromosome:chromosome 1
Location:808096
Confidence (%):80
Locus systematic id Locus common name Defline Feature
Cre01.g004450 CPLD42 (1 of 5) PF07466 - Protein of unknown function (DUF1517) (DUF1517); conserved expressed membrane protein CDS

Insertion site details

Expected flanking sequence (starting at cassette; orientation from cassette outwards): CGCACGGCTTAAAGGCGCGGGCGGCGGAACGCGCGCTCACAGCCGGCGTG
Internal bar code:TCTATCGGAATAGTCGGACTGG

Confirmation - LEAP-Seq

LEAP-Seq distance:866
LEAP-Seq percent confirming:100.0
LEAP-Seq n confirming:6
LEAP-Seq n nonconfirming:0
LEAP-Seq n unique pos:6

Suggested primers for confirmation by PCR

Suggested primer 1:GTGTGTGTTCGGTCACGTTG
Suggested primer 2:TCACCTGCACCTTCATGACC