Insertion junction: CLIP2.003247_1

Overview

Insertion cassette:CIB2
Side of cassette:3'
Strand:-
Strain:CLIP2.003247
Chromosome:chromosome 6
Location:5569676
Confidence (%):80
Locus systematic id Locus common name Defline Feature
Cre06.g287176 CPLD67 dihydrolipoyllysine-residue acetyltransferase; (1 of 5) PF01713 - Smr domain (Smr) intron

Insertion site details

Expected flanking sequence (starting at cassette; orientation from cassette outwards): TTGAAATGGCTAACCCCCTTCCCCACGCAACCCACAAGCACTCACCAAAT
Internal bar code:TACGTGTAGGCTCTTTATACAT

Confirmation - LEAP-Seq

LEAP-Seq distance:4000
LEAP-Seq percent confirming:100.0
LEAP-Seq n confirming:28
LEAP-Seq n nonconfirming:0
LEAP-Seq n unique pos:28

Suggested primers for confirmation by PCR

Suggested primer 1:GCAAGAGGAGATCGAGGAGC
Suggested primer 2:AGTCCTCCAAAAGGCTGTCG