Insertion junction: CLIP2.003254_1

Overview

Insertion cassette:CIB2
Side of cassette:5' truncated?
Strand:-
Strain:CLIP2.003254
Chromosome:chromosome 4
Location:3761688
Confidence (%):80
Locus systematic id Locus common name Defline Feature
Cre04.g229494 (1 of 70) 3.1.3.16 - Protein-serine/threonine phosphatase / Serine/threonine specific protein phosphatase intron

Insertion site details

Expected flanking sequence (starting at cassette; orientation from cassette outwards): CCCCCGCACCTGAACACTCGCCGCAGAGCCCCCGCCACTGCCTCCTCCTC
Internal bar code:ATGGCTCCCATACAACGTCTCT

Confirmation - LEAP-Seq

LEAP-Seq distance:364
LEAP-Seq percent confirming:91.6667
LEAP-Seq n confirming:22
LEAP-Seq n nonconfirming:2
LEAP-Seq n unique pos:24

Suggested primers for confirmation by PCR

Suggested primer 1:CTATACCAAGCCGGGCTGAG
Suggested primer 2:ACCAGCCCAAACTGAACCAA