| Insertion cassette: | CIB2 |
| Side of cassette: | 5' |
| Strand: | + |
| Strain: | CLIP2.003261 |
| Chromosome: | chromosome 1 |
| Location: | 1974001 |
| Confidence (%): | 80 |
| Locus systematic id | Locus common name | Defline | Feature |
|---|---|---|---|
| Cre01.g010864 | (1 of 1) K02836 - peptide chain release factor 2 (prfB); Homolog to bacterial PrfB peptide chain release factor | CDS |
Insertion site details | |
| Expected flanking sequence (starting at cassette; orientation from cassette outwards): | GTCGCAGGCGCAGAACAAGGCCATTGCCCTGGACCTGCTCAAGGTGGGCG |
| Internal bar code: | GTGATAAGTGCGTTAAAGGTAG |
Confirmation - LEAP-Seq | |
| LEAP-Seq distance: | 287 |
| LEAP-Seq percent confirming: | 78.2609 |
| LEAP-Seq n confirming: | 18 |
| LEAP-Seq n nonconfirming: | 5 |
| LEAP-Seq n unique pos: | 23 |
Suggested primers for confirmation by PCR | |
| Suggested primer 1: | CACCAACTTGTACGGGTGGA |
| Suggested primer 2: | CTTGTCTGCCACGACCTCTT |