| Insertion cassette: | CIB2 |
| Side of cassette: | 5' |
| Strand: | + |
| Strain: | CLIP2.003268 |
| Chromosome: | chromosome 10 |
| Location: | 3285350 |
| Confidence (%): | 80 |
| Locus systematic id | Locus common name | Defline | Feature |
|---|---|---|---|
| Cre10.g442950 | (1 of 2) PF13483 - Beta-lactamase superfamily domain (Lactamase_B_3) | 3'UTR |
Insertion site details | |
| Expected flanking sequence (starting at cassette; orientation from cassette outwards): | CACGATGTGGCGGTGGTGGATACACCTGCAGCGGTGGAGCGGATGTGTGA |
| Internal bar code: | GTTGGATGCATCGGGCCAGATC |
Confirmation - LEAP-Seq | |
| LEAP-Seq distance: | 1707 |
| LEAP-Seq percent confirming: | 96.3636 |
| LEAP-Seq n confirming: | 53 |
| LEAP-Seq n nonconfirming: | 2 |
| LEAP-Seq n unique pos: | 55 |
Suggested primers for confirmation by PCR | |
| Suggested primer 1: | CCCTCAAGTGTTGCACAAGC |
| Suggested primer 2: | TTTGCAGCTGTCTTTGCGTC |