| Insertion cassette: | CIB2 |
| Side of cassette: | 5' truncated? |
| Strand: | + |
| Strain: | CLIP2.003286 |
| Chromosome: | chromosome 1 |
| Location: | 808512 |
| Confidence (%): | 80 |
| Locus systematic id | Locus common name | Defline | Feature |
|---|---|---|---|
| Cre01.g004450 | CPLD42 | (1 of 5) PF07466 - Protein of unknown function (DUF1517) (DUF1517); conserved expressed membrane protein | 5'UTR_intron |
Insertion site details | |
| Expected flanking sequence (starting at cassette; orientation from cassette outwards): | TGTGACACAACATGGGTATAGCTGTGATGACGTGCGGGAGCCTACCGACA |
| Internal bar code: | AGGGGTTTACTCAAAGTCAGCG |
Confirmation - LEAP-Seq | |
| LEAP-Seq distance: | 1748 |
| LEAP-Seq percent confirming: | 96.0 |
| LEAP-Seq n confirming: | 24 |
| LEAP-Seq n nonconfirming: | 1 |
| LEAP-Seq n unique pos: | 25 |
Suggested primers for confirmation by PCR | |
| Suggested primer 1: | GTGTGTGTTCGGTCACGTTG |
| Suggested primer 2: | AGGCTGACGTCTCAATTGGG |