| Insertion cassette: | CIB2 |
| Side of cassette: | 5' |
| Strand: | + |
| Strain: | CLIP2.003293 |
| Chromosome: | chromosome 10 |
| Location: | 4674239 |
| Confidence (%): | 80 |
| Locus systematic id | Locus common name | Defline | Feature |
|---|---|---|---|
| Cre10.g452050 | LHCA4 | (1 of 1) K08908 - light-harvesting complex I chlorophyll a/b binding protein 2 (LHCA2); light-harvesting protein of photosystem I | intron |
Insertion site details | |
| Expected flanking sequence (starting at cassette; orientation from cassette outwards): | AGTTCGTGGATACGGTCCGCTCACGTGGCGAGCCCTGCCTCCCATATGCA |
| Internal bar code: | AATGTTGCGAGCAAGGCCTCCA |
Confirmation - LEAP-Seq | |
| LEAP-Seq distance: | 3256 |
| LEAP-Seq percent confirming: | 100.0 |
| LEAP-Seq n confirming: | 77 |
| LEAP-Seq n nonconfirming: | 0 |
| LEAP-Seq n unique pos: | 77 |
Suggested primers for confirmation by PCR | |
| Suggested primer 1: | TCACACAGGACTTCGGCTTC |
| Suggested primer 2: | TTCTGAAGTGCAGCCGTCAT |