Insertion junction: CLIP2.003294_2

Overview

Insertion cassette:CIB2
Side of cassette:5'
Strand:+
Strain:CLIP2.003294
Chromosome:chromosome 16
Location:4603256
Confidence (%):80
Locus systematic id Locus common name Defline Feature
Cre16.g670950 CYC4 c-type cytochrome, chloroplast precursor; (1 of 1) PTHR34688//PTHR34688:SF1 - FAMILY NOT NAMED // CYTOCHROME C6, CHLOROPLASTIC intron

Insertion site details

Expected flanking sequence (starting at cassette; orientation from cassette outwards): CCCGGCACGGACGGCGCAGAGATAGCTACATCAGCTAGTTTGTCTGCAGT
Internal bar code:TAGGGAGCTTCTGGATGCCGTG

Confirmation - LEAP-Seq

LEAP-Seq distance:2084
LEAP-Seq percent confirming:100.0
LEAP-Seq n confirming:45
LEAP-Seq n nonconfirming:0
LEAP-Seq n unique pos:45

Suggested primers for confirmation by PCR

Suggested primer 1:TCACCTCCTCATCGCTCAGA
Suggested primer 2:CGAAAACGCTTGAAAGGCCA