| Insertion cassette: | CIB2 |
| Side of cassette: | 5' |
| Strand: | - |
| Strain: | CLIP2.003296 |
| Chromosome: | chromosome 2 |
| Location: | 2330428 |
| Confidence (%): | 80 |
| Locus systematic id | Locus common name | Defline | Feature |
|---|---|---|---|
| Cre02.g090650 | SEC20 | (1 of 1) K08497 - protein transport protein SEC20 (SEC20); Qb-SNARE protein, Sec20-family | CDS |
Insertion site details | |
| Expected flanking sequence (starting at cassette; orientation from cassette outwards): | GTCGTCCGCAAGGTGACTGCGCAGCCACCAGGGCAGGGCACTCACGCCCT |
| Internal bar code: | GTTATGCGCTTAACTATACACT |
Confirmation - LEAP-Seq | |
| LEAP-Seq distance: | 3567 |
| LEAP-Seq percent confirming: | 70.5882 |
| LEAP-Seq n confirming: | 12 |
| LEAP-Seq n nonconfirming: | 5 |
| LEAP-Seq n unique pos: | 17 |
Suggested primers for confirmation by PCR | |
| Suggested primer 1: | CTTGCAGACGACCCTGAGTT |
| Suggested primer 2: | TCTTTAGCCAACGCCTCCTG |