Insertion junction: CLIP2.003342_1

Overview

Insertion cassette:CIB2
Side of cassette:3'
Strand:-
Strain:CLIP2.003342
Chromosome:chromosome 12
Location:4333558
Confidence (%):80
Locus systematic id Locus common name Defline Feature
Cre12.g519000 ASB2,PAI1 Phosphoribosylanthranilate isomerase 1; (1 of 1) 5.3.1.24 - Phosphoribosylanthranilate isomerase / PRAI intron

Insertion site details

Expected flanking sequence (starting at cassette; orientation from cassette outwards): GTGGCGTGGGCCACACACGCCTCGGGGAGCCGCCACCAGGTCTGCCCCTG
Internal bar code:TCGTAGCGAGTCCTCGAGAAGA

Confirmation - LEAP-Seq

LEAP-Seq distance:1580
LEAP-Seq percent confirming:100.0
LEAP-Seq n confirming:3
LEAP-Seq n nonconfirming:0
LEAP-Seq n unique pos:3

Suggested primers for confirmation by PCR

Suggested primer 1:CGAGGTGACAGCCAGTTCTT
Suggested primer 2:GCCAGCGGATGCAGTAGTAT