Insertion junction: CLIP2.003344_1

Overview

Insertion cassette:CIB2
Side of cassette:5'
Strand:+
Strain:CLIP2.003344
Chromosome:chromosome 16
Location:6153010
Confidence (%):80
Locus systematic id Locus common name Defline Feature
Cre16.g677050 (1 of 7) PF00211//PF13416 - Adenylate and Guanylate cyclase catalytic domain (Guanylate_cyc) // Bacterial extracellular solute-binding protein (SBP_bac_8) intron

Insertion site details

Expected flanking sequence (starting at cassette; orientation from cassette outwards): AACCCCAACCACACCCCAAGATTGCCTACCATCTACCACTTTCTGAACTG
Internal bar code:TATGAGAGAGAATCGGGGGCAA

Confirmation - LEAP-Seq

LEAP-Seq distance:63
LEAP-Seq percent confirming:75.0
LEAP-Seq n confirming:3
LEAP-Seq n nonconfirming:1
LEAP-Seq n unique pos:4

Suggested primers for confirmation by PCR

Suggested primer 1:TGGAGCTTAGTCCCTCCCTC
Suggested primer 2:ATCCAAGGCATGTGGTCCTG