Insertion junction: CLIP2.003346_1

Overview

Insertion cassette:CIB2
Side of cassette:3'
Strand:-
Strain:CLIP2.003346
Chromosome:chromosome 1
Location:4437295
Confidence (%):80
Locus systematic id Locus common name Defline Feature
Cre01.g030250 (1 of 1) 3.1.3.57 - Inositol-1,4-bisphosphate 1-phosphatase / Inositol polyphosphate 1-phosphatase 5'UTR
Cre01.g030300 (1 of 2) PF08627 - CRT-like, chloroquine-resistance transporter-like (CRT-like) CDS

Insertion site details

Expected flanking sequence (starting at cassette; orientation from cassette outwards): ACTTCCACATGGACACATGGCCGGCTTCTTGGGCCCTTCAGCGCGCCCGG
Internal bar code:GATTGGGGGACACGATCGTAGT

Confirmation - LEAP-Seq

LEAP-Seq distance:3330
LEAP-Seq percent confirming:100.0
LEAP-Seq n confirming:98
LEAP-Seq n nonconfirming:0
LEAP-Seq n unique pos:98

Suggested primers for confirmation by PCR

Suggested primer 1:ACGGCCTGGACAGCAAATTA
Suggested primer 2:GGTAGAGGAGGACGTGAGGT