| Insertion cassette: | CIB2 |
| Side of cassette: | 5' |
| Strand: | + |
| Strain: | CLIP2.003401 |
| Chromosome: | chromosome 4 |
| Location: | 2041408 |
| Confidence (%): | 80 |
| Locus systematic id | Locus common name | Defline | Feature |
|---|---|---|---|
| Cre04.g219200 | CPL19 | (1 of 1) IPR007751//IPR015422//IPR029058 - Domain of unknown function DUF676, lipase-like // Pyridoxal phosphate-dependent transferase, major region, subdomain 2 // Alpha/Beta hydrolase fold; possible serine esterase | 3'UTR_intron |
Insertion site details | |
| Expected flanking sequence (starting at cassette; orientation from cassette outwards): | TGGGGTGTGAGGCATGGCGTATGAGGCATGGGGTGTGAGGCATGGGGTGT |
| Internal bar code: | GAATGCGTCAAATCGCCAAGTT |
Confirmation - LEAP-Seq | |
| LEAP-Seq distance: | 1028 |
| LEAP-Seq percent confirming: | 90.625 |
| LEAP-Seq n confirming: | 29 |
| LEAP-Seq n nonconfirming: | 3 |
| LEAP-Seq n unique pos: | 32 |
Suggested primers for confirmation by PCR | |
| Suggested primer 1: | CATGCGCGAGTGTGTACATG |
| Suggested primer 2: | CCATATGCTAACCCGCCCAT |