| Insertion cassette: | CIB2 |
| Side of cassette: | 5' truncated? |
| Strand: | + |
| Strain: | CLIP2.003405 |
| Chromosome: | chromosome 1 |
| Location: | 4862222 |
| Confidence (%): | 63 |
| Locus systematic id | Locus common name | Defline | Feature |
|---|---|---|---|
| Cre01.g033832 | HEL7,RH39 | Possible DEAD-box helicase involved in creation of hidden rRNA breaks in chloroplast ribosomes; (1 of 1) PTHR24031//PTHR24031:SF247 - RNA HELICASE // DEAD-BOX ATP-DEPENDENT RNA HELICASE 39 | intron |
Insertion site details | |
| Expected flanking sequence (starting at cassette; orientation from cassette outwards): | CATCCACCACCCTATCCACCCGCCCACACGCATGCATACACACACATTGC |
| Internal bar code: | GACGTACTGAGCCGAAATATCC |
Confirmation - LEAP-Seq | |
| LEAP-Seq distance: | 164 |
| LEAP-Seq percent confirming: | 100.0 |
| LEAP-Seq n confirming: | 11 |
| LEAP-Seq n nonconfirming: | 0 |
| LEAP-Seq n unique pos: | 11 |
Suggested primers for confirmation by PCR | |
| Suggested primer 1: | GGAGACACACATCCACTGCA |
| Suggested primer 2: | ACGCACACACAAACACACAC |