| Insertion cassette: | CIB2 |
| Side of cassette: | 5' |
| Strand: | + |
| Strain: | CLIP2.003409 |
| Chromosome: | chromosome 5 |
| Location: | 1578513 |
| Confidence (%): | 80 |
| Locus systematic id | Locus common name | Defline | Feature |
|---|---|---|---|
| Cre05.g234750 | CGLD25 | Conserved in the Green Lineage and Diatoms | 3'UTR |
Insertion site details | |
| Expected flanking sequence (starting at cassette; orientation from cassette outwards): | GATCCCCGTGAGTACTCGTGCTGGCAAAATTCCTACACCCACAGAAGTAT |
| Internal bar code: | TCTTGGTACGCAATAGCTCCGC |
Confirmation - LEAP-Seq | |
| LEAP-Seq distance: | 2796 |
| LEAP-Seq percent confirming: | 75.0 |
| LEAP-Seq n confirming: | 27 |
| LEAP-Seq n nonconfirming: | 9 |
| LEAP-Seq n unique pos: | 36 |
Suggested primers for confirmation by PCR | |
| Suggested primer 1: | CACAAAACCGTGAACGACCC |
| Suggested primer 2: | GGAACACGAGCCTTGTCTGA |