| Insertion cassette: | CIB2 |
| Side of cassette: | 3' truncated? |
| Strand: | - |
| Strain: | CLIP2.003417 |
| Chromosome: | chromosome 2 |
| Location: | 7716694 |
| Confidence (%): | 80 |
| Locus systematic id | Locus common name | Defline | Feature |
|---|---|---|---|
| Cre02.g146950 | DBR1 | RNA lariat debranching enzyme; (1 of 1) K18328 - lariat debranching enzyme [EC:3.1.-.-] (DBR1) | intron |
Insertion site details | |
| Expected flanking sequence (starting at cassette; orientation from cassette outwards): | ACCCAAGCCCGTCCTGTCTTGCACACACCTCGCGACGCCGTGCCTCCAAA |
| Internal bar code: | TATCCCGATACGAGCAACAAAG |
Confirmation - LEAP-Seq | |
| LEAP-Seq distance: | 750 |
| LEAP-Seq percent confirming: | 88.8889 |
| LEAP-Seq n confirming: | 8 |
| LEAP-Seq n nonconfirming: | 1 |
| LEAP-Seq n unique pos: | 9 |
Suggested primers for confirmation by PCR | |
| Suggested primer 1: | AGTGTCGTGTTGTGTTTGCG |
| Suggested primer 2: | TGGACATTCACTCGTCGCAA |