| Insertion cassette: | CIB2 |
| Side of cassette: | 5' |
| Strand: | - |
| Strain: | CLIP2.003422 |
| Chromosome: | chromosome 2 |
| Location: | 6062264 |
| Confidence (%): | 80 |
| Locus systematic id | Locus common name | Defline | Feature |
|---|---|---|---|
| Cre02.g119100 | CER2 | (1 of 2) K15889 - prenylcysteine alpha-carboxyl methylesterase (PCME); Carboxyesterase-related protein/arylformamidase | intron |
Insertion site details | |
| Expected flanking sequence (starting at cassette; orientation from cassette outwards): | TCGACGAAATGAGTTAGGTTTAGTAAGCATAGTGACGCTACAGGCAGGCG |
| Internal bar code: | ATACGATTTTACTCTAGGCCAT |
Confirmation - LEAP-Seq | |
| LEAP-Seq distance: | 5159 |
| LEAP-Seq percent confirming: | 96.9697 |
| LEAP-Seq n confirming: | 32 |
| LEAP-Seq n nonconfirming: | 1 |
| LEAP-Seq n unique pos: | 33 |
Suggested primers for confirmation by PCR | |
| Suggested primer 1: | GTACGGCGACAAGGTGTGTA |
| Suggested primer 2: | ATGATGGCAGAGGACACAGC |