| Insertion cassette: | CIB2 |
| Side of cassette: | 5' |
| Strand: | + |
| Strain: | CLIP2.003425 |
| Chromosome: | chromosome 6 |
| Location: | 2643734 |
| Confidence (%): | 80 |
| Locus systematic id | Locus common name | Defline | Feature |
|---|---|---|---|
| Cre06.g270300 | (1 of 1) PTHR13271//PTHR13271:SF36 - UNCHARACTERIZED PUTATIVE METHYLTRANSFERASE // SUBFAMILY NOT NAMED | 3'UTR | |
| Cre06.g800644 | gene_edge/mRNA_edge/5'UTR |
Insertion site details | |
| Expected flanking sequence (starting at cassette; orientation from cassette outwards): | GGGACGAAAGGCACTCTCGGGGCCAACCACCTTTCCGTACCTCATGCCGC |
| Internal bar code: | GTCTGTTATCAGCGTGACCCCA |
Confirmation - LEAP-Seq | |
| LEAP-Seq distance: | 4619 |
| LEAP-Seq percent confirming: | 94.382 |
| LEAP-Seq n confirming: | 84 |
| LEAP-Seq n nonconfirming: | 5 |
| LEAP-Seq n unique pos: | 89 |
Suggested primers for confirmation by PCR | |
| Suggested primer 1: | TGCTTGAGTCAGACCTGCAG |
| Suggested primer 2: | GTGACACGAGGAAGTCAGCA |