| Insertion cassette: | CIB2 |
| Side of cassette: | 3' |
| Strand: | + |
| Strain: | CLIP2.003445 |
| Chromosome: | chromosome 3 |
| Location: | 7272159 |
| Confidence (%): | 80 |
| Locus systematic id | Locus common name | Defline | Feature |
|---|---|---|---|
| Cre03.g207400 | (1 of 1) PTHR36846:SF1 - PROTEIN VIAA | outside_mRNA |
Insertion site details | |
| Expected flanking sequence (starting at cassette; orientation from cassette outwards): | AGGGGTGTCGTCCTAACGGATGCGTGGGGACATGCCAACAAGTCCACACG |
| Internal bar code: | CACTTTGCGCCAATCCCGTGTT |
Confirmation - LEAP-Seq | |
| LEAP-Seq distance: | 2252 |
| LEAP-Seq percent confirming: | 92.8571 |
| LEAP-Seq n confirming: | 13 |
| LEAP-Seq n nonconfirming: | 1 |
| LEAP-Seq n unique pos: | 14 |
Suggested primers for confirmation by PCR | |
| Suggested primer 1: | CTCCTGCTTCCATGCCTTCA |
| Suggested primer 2: | AATTCTGAGCAGGGCAGACC |