| Insertion cassette: | CIB2 |
| Side of cassette: | 5' |
| Strand: | + |
| Strain: | CLIP2.003457 |
| Chromosome: | chromosome 2 |
| Location: | 5006318 |
| Confidence (%): | 80 |
| Locus systematic id | Locus common name | Defline | Feature |
|---|---|---|---|
| Cre02.g110750 | LHCB7,LHCQ | Light-harvesting chlorophyll a-b binding protein, chloroplast precursor; (1 of 1) K14172 - light-harvesting complex II chlorophyll a/b binding protein 7 (LHCB7) | 3'UTR |
| Cre02.g110800 | NRT2F | Nitrate/nitrite transporter; (1 of 4) K02575 - MFS transporter, NNP family, nitrate/nitrite transporter (NRT, narK, nrtP, nasA) | 5'UTR |
Insertion site details | |
| Expected flanking sequence (starting at cassette; orientation from cassette outwards): | TTTCGGCAGGCTGCGGGCAAGCGCAGATACGGGCAACACATGGCCCGTCA |
| Internal bar code: | GTCTCCTCGATTTGTCGAACTG |
Confirmation - LEAP-Seq | |
| LEAP-Seq distance: | 3518 |
| LEAP-Seq percent confirming: | 100.0 |
| LEAP-Seq n confirming: | 77 |
| LEAP-Seq n nonconfirming: | 0 |
| LEAP-Seq n unique pos: | 77 |
Suggested primers for confirmation by PCR | |
| Suggested primer 1: | GCAAGTTGCCAAGTCCAAGG |
| Suggested primer 2: | AGGAGAGTCACAACTGTGCG |