Insertion junction: CLIP2.003465_1

Overview

Insertion cassette:CIB2
Side of cassette:5'
Strand:-
Strain:CLIP2.003465
Chromosome:chromosome 14
Location:3912251
Confidence (%):80
Locus systematic id Locus common name Defline Feature
Cre14.g632775 (1 of 1) K03035 - 26S proteasome regulatory subunit N5 (PSMD12, RPN5) intron

Insertion site details

Expected flanking sequence (starting at cassette; orientation from cassette outwards): GAGGTCCCCTCCTTCCACGTGGAACACACAACCGCTAGACGCCGGCACTT
Internal bar code:AACGGTAAAGTGTGATTGCGGC

Confirmation - LEAP-Seq

LEAP-Seq distance:317
LEAP-Seq percent confirming:53.3333
LEAP-Seq n confirming:8
LEAP-Seq n nonconfirming:7
LEAP-Seq n unique pos:15

Suggested primers for confirmation by PCR

Suggested primer 1:AACACCGACTCGCTTATGCA
Suggested primer 2:GGCGGCATCACAAAAACACT