| Insertion cassette: | CIB2 |
| Side of cassette: | 5' |
| Strand: | - |
| Strain: | CLIP2.003466 |
| Chromosome: | chromosome 13 |
| Location: | 1805732 |
| Confidence (%): | 80 |
| Locus systematic id | Locus common name | Defline | Feature |
|---|---|---|---|
| Cre13.g575000 | CCS1 | c-type cytochrome synthesis 1; (1 of 1) K07399 - cytochrome c biogenesis protein (resB, ccs1) | 3'UTR |
Insertion site details | |
| Expected flanking sequence (starting at cassette; orientation from cassette outwards): | GTCCAGGCGACCAGGGTGCATTTGGGGCGTGCGGCGATGCGCTGCTGCCG |
| Internal bar code: | AAGTCAGCAGAAGTTCCTTGGA |
Confirmation - LEAP-Seq | |
| LEAP-Seq distance: | 5022 |
| LEAP-Seq percent confirming: | 76.1364 |
| LEAP-Seq n confirming: | 67 |
| LEAP-Seq n nonconfirming: | 21 |
| LEAP-Seq n unique pos: | 88 |
Suggested primers for confirmation by PCR | |
| Suggested primer 1: | ATGCTGGGAGTGTGTCAGTG |
| Suggested primer 2: | GGGTAGGGTGGGTGCTAGTA |