Insertion junction: CLIP2.003466_2

Overview

Insertion cassette:CIB2
Side of cassette:3'
Strand:+
Strain:CLIP2.003466
Chromosome:chromosome 13
Location:1805746
Confidence (%):80
Locus systematic id Locus common name Defline Feature
Cre13.g575000 CCS1 c-type cytochrome synthesis 1; (1 of 1) K07399 - cytochrome c biogenesis protein (resB, ccs1) 3'UTR

Insertion site details

Expected flanking sequence (starting at cassette; orientation from cassette outwards): CGCCCATCGCGCCAGACCAGTATTCGTAAGCCGCCATCATCAAAAGGGTG
Internal bar code:CTTTTTCGATCTGAGGTGTTTA

Confirmation - LEAP-Seq

LEAP-Seq distance:5619
LEAP-Seq percent confirming:100.0
LEAP-Seq n confirming:62
LEAP-Seq n nonconfirming:0
LEAP-Seq n unique pos:62

Suggested primers for confirmation by PCR

Suggested primer 1:GGGTAGGGTGGGTGCTAGTA
Suggested primer 2:ATGCTGGGAGTGTGTCAGTG