Insertion junction: CLIP2.003486_2

Overview

Insertion cassette:CIB2
Side of cassette:3' truncated?
Strand:+
Strain:CLIP2.003486
Chromosome:chromosome 7
Location:4108449
Confidence (%):80
Locus systematic id Locus common name Defline Feature
Cre07.g342800 CGL16 (1 of 1) PF07063 - Domain of unknown function (DUF1338) (DUF1338); Conserved in the Green Lineage CDS

Insertion site details

Expected flanking sequence (starting at cassette; orientation from cassette outwards): CAAGAAGCTGGCCGCCACCTGGTTCCGGGCGGCGGACCCCGAGGCGCGGC
Internal bar code:TCGGGTGACGGTCTGGTACTCA

Confirmation - LEAP-Seq

LEAP-Seq distance:1059
LEAP-Seq percent confirming:92.8571
LEAP-Seq n confirming:13
LEAP-Seq n nonconfirming:1
LEAP-Seq n unique pos:14

Suggested primers for confirmation by PCR

Suggested primer 1:GGGGACCGTGTGTGTACATT
Suggested primer 2:CAAATGGCACTGTTCCGCAA