Insertion junction: CLIP2.003503_2

Overview

Insertion cassette:CIB2
Side of cassette:5'
Strand:-
Strain:CLIP2.003503
Chromosome:chromosome 6
Location:3950771
Confidence (%):80
Locus systematic id Locus common name Defline Feature
Cre06.g278199 (1 of 4) 1.13.11.75 - All-trans-8'-apo-beta-carotenal 15,15'-oxygenase / 8'-apo-beta-carotenal 15,15'-oxygenase CDS

Insertion site details

Expected flanking sequence (starting at cassette; orientation from cassette outwards): GCGGGCTGCAGGCCCAGGTGGGCGGGGTGGGGCAGGACAGGGAGGTGTGT
Internal bar code:AAAGTATTGCAGGTTTCGTTCA

Confirmation - LEAP-Seq

LEAP-Seq distance:1507
LEAP-Seq percent confirming:100.0
LEAP-Seq n confirming:25
LEAP-Seq n nonconfirming:0
LEAP-Seq n unique pos:25

Suggested primers for confirmation by PCR

Suggested primer 1:CGTGTCCATGTCCACCTTCA
Suggested primer 2:TCTGCCTCTCTGCTTTCTGC