| Insertion cassette: | CIB2 |
| Side of cassette: | 5' |
| Strand: | + |
| Strain: | CLIP2.003504 |
| Chromosome: | chromosome 16 |
| Location: | 519346 |
| Confidence (%): | 80 |
| Locus systematic id | Locus common name | Defline | Feature |
|---|---|---|---|
| Cre16.g692650 | CGL4 | Conserved in the Green Lineage; (1 of 173) IPR029058 - Alpha/Beta hydrolase fold | CDS |
Insertion site details | |
| Expected flanking sequence (starting at cassette; orientation from cassette outwards): | TGGTGGCCTCATCCACCTCAGGGGCACAGCGCTGGAGGACAGCGGCATGG |
| Internal bar code: | ACCGTGTTCTAGTTGCAAGGTT |
Confirmation - LEAP-Seq | |
| LEAP-Seq distance: | 485 |
| LEAP-Seq percent confirming: | 72.093 |
| LEAP-Seq n confirming: | 31 |
| LEAP-Seq n nonconfirming: | 12 |
| LEAP-Seq n unique pos: | 43 |
Suggested primers for confirmation by PCR | |
| Suggested primer 1: | GGGGTGTAGGAAGGGGAGAT |
| Suggested primer 2: | CACCAGTTTGTCCTTGCAGC |