| Insertion cassette: | CIB2 |
| Side of cassette: | 5' |
| Strand: | + |
| Strain: | CLIP2.003509 |
| Chromosome: | chromosome 8 |
| Location: | 3125612 |
| Confidence (%): | 80 |
| Locus systematic id | Locus common name | Defline | Feature |
|---|---|---|---|
| Cre08.g375950 | EXR1 | (1 of 1) PF13474 - SnoaL-like domain (SnoaL_3); UvrB containing protein | CDS |
Insertion site details | |
| Expected flanking sequence (starting at cassette; orientation from cassette outwards): | GACAGCTGGCGCGCCATCATGCGGAATGTGCGGCCGGGCGCCTTCAAGGT |
| Internal bar code: | CACTTATCTGACACTGCGGAAG |
Confirmation - LEAP-Seq | |
| LEAP-Seq distance: | 1170 |
| LEAP-Seq percent confirming: | 70.0 |
| LEAP-Seq n confirming: | 7 |
| LEAP-Seq n nonconfirming: | 3 |
| LEAP-Seq n unique pos: | 10 |
Suggested primers for confirmation by PCR | |
| Suggested primer 1: | CCTTCTATGCGTGCTAGCGA |
| Suggested primer 2: | ATATGGGACTGGAGGGCTGT |