Insertion junction: CLIP2.003546_2

Overview

Insertion cassette:CIB2
Side of cassette:3' truncated?
Strand:-
Strain:CLIP2.003546
Chromosome:chromosome 6
Location:4902165
Confidence (%):80
Locus systematic id Locus common name Defline Feature
Cre06.g280650 CGL59,Y3IP1 Chloroplast Ycf3 interacting protein involved in Photosystem I biogensis 5'UTR

Insertion site details

Expected flanking sequence (starting at cassette; orientation from cassette outwards): CTTCATATTCTAATAATAAATCTAACACATTCAATCTATTGGATATCACT
Internal bar code:GTATAGTTGTAATGAGACTCGT

Confirmation - LEAP-Seq

LEAP-Seq distance:213
LEAP-Seq percent confirming:22.2222
LEAP-Seq n confirming:2
LEAP-Seq n nonconfirming:7
LEAP-Seq n unique pos:9

Suggested primers for confirmation by PCR

Suggested primer 1:GAGGTGTGTGAGGGTCTTCG
Suggested primer 2:CCTCAAAAATGTCCGCGCAA