Insertion junction: CLIP2.003567_2

Overview

Insertion cassette:CIB2
Side of cassette:5' truncated?
Strand:-
Strain:CLIP2.003567
Chromosome:chromosome 12
Location:4175254
Confidence (%):80
Locus systematic id Locus common name Defline Feature
Cre12.g517700 NYC1,SDR21 (1 of 2) K13606 - chlorophyll(ide) b reductase (NOL, NYC1); Chlorophyll b reductase intron

Insertion site details

Expected flanking sequence (starting at cassette; orientation from cassette outwards): GAATGTGTTGTGTGTGGCGAGGGTTCAAGAGGAGGCTAGAAGCACAAGCG
Internal bar code:CTATTCGACCTCAAGTTCCAAC

Confirmation - LEAP-Seq

LEAP-Seq distance:1218
LEAP-Seq percent confirming:73.6842
LEAP-Seq n confirming:14
LEAP-Seq n nonconfirming:5
LEAP-Seq n unique pos:19

Suggested primers for confirmation by PCR

Suggested primer 1:CTGCTCTCCCAATTTGCTGC
Suggested primer 2:AGCACACTCAGGTTGTAGGC