| Insertion cassette: | CIB2 |
| Side of cassette: | 5' |
| Strand: | - |
| Strain: | CLIP2.003573 |
| Chromosome: | chromosome 6 |
| Location: | 5729679 |
| Confidence (%): | 80 |
| Locus systematic id | Locus common name | Defline | Feature |
|---|---|---|---|
| Cre06.g288350 | 5'UTR | ||
| lncRNA_TCONS_00144277 | 5'UTR |
Insertion site details | |
| Expected flanking sequence (starting at cassette; orientation from cassette outwards): | ATTGAGAAAGCGACTAGAATCCCATTGCCTGGGCCTCCCAAGGCATGATA |
| Internal bar code: | ACTCTGAACTGAGGAATTGTCA |
Confirmation - LEAP-Seq | |
| LEAP-Seq distance: | 1076 |
| LEAP-Seq percent confirming: | 73.0769 |
| LEAP-Seq n confirming: | 19 |
| LEAP-Seq n nonconfirming: | 7 |
| LEAP-Seq n unique pos: | 26 |
Suggested primers for confirmation by PCR | |
| Suggested primer 1: | TTGATAGCTCGATTCGGCCC |
| Suggested primer 2: | GAGTTGATGCGGCGAATCAC |