Insertion junction: CLIP2.003576_1

Overview

Insertion cassette:CIB2
Side of cassette:5'
Strand:-
Strain:CLIP2.003576
Chromosome:chromosome 2
Location:4727630
Confidence (%):80
Locus systematic id Locus common name Defline Feature
Cre02.g108300 intron

Insertion site details

Expected flanking sequence (starting at cassette; orientation from cassette outwards): ACAGCCTGGCGCACGCACCCACAATCCCGCATACACCACACTCCACCTCC
Internal bar code:AATCCGCCACCGCCGCCAGGCT

Confirmation - LEAP-Seq

LEAP-Seq distance:2328
LEAP-Seq percent confirming:88.8889
LEAP-Seq n confirming:8
LEAP-Seq n nonconfirming:1
LEAP-Seq n unique pos:9

Suggested primers for confirmation by PCR

Suggested primer 1:CTCCCCTCCATCTGTACCCA
Suggested primer 2:TCTCCTTGTCCTCCTCCTCG