| Insertion cassette: | CIB2 |
| Side of cassette: | 5' truncated? |
| Strand: | + |
| Strain: | CLIP2.003585 |
| Chromosome: | chromosome 7 |
| Location: | 3291870 |
| Confidence (%): | 80 |
| Locus systematic id | Locus common name | Defline | Feature |
|---|---|---|---|
| Cre07.g334550 | PSAO,PSAO1 | Photosystem I reaction center subunit O; (1 of 1) K14332 - photosystem I subunit PsaO (psaO) | 5'UTR |
Insertion site details | |
| Expected flanking sequence (starting at cassette; orientation from cassette outwards): | CGTGCAGATGACTTTGGCCGGGCTCCCAAAGCGCTTGCATCGGCGCTCGA |
| Internal bar code: | TAGTACCATCCCCTCCAGCTTC |
Confirmation - LEAP-Seq | |
| LEAP-Seq distance: | 1044 |
| LEAP-Seq percent confirming: | 85.0 |
| LEAP-Seq n confirming: | 17 |
| LEAP-Seq n nonconfirming: | 3 |
| LEAP-Seq n unique pos: | 20 |
Suggested primers for confirmation by PCR | |
| Suggested primer 1: | CTCTACCTACTCAGTGCGCG |
| Suggested primer 2: | GTTCTCGCCGATAGACTGGG |