Insertion junction: CLIP2.003596_2

Overview

Insertion cassette:CIB2
Side of cassette:3'
Strand:+
Strain:CLIP2.003596
Chromosome:chromosome 1
Location:5307449
Confidence (%):80
Locus systematic id Locus common name Defline Feature
Cre01.g037350 TEH2,CGLD2 Thioesterase family protein; (1 of 2) K17361 - acyl-coenzyme A thioesterase 9 [EC:3.1.2.-] (ACOT9) intron

Insertion site details

Expected flanking sequence (starting at cassette; orientation from cassette outwards): CTTGTTGGGCTCGGGCATACCACCCACTCACCCACCCACCCAAGCACCCG
Internal bar code:CACAGTTTGTTTTAGGTATACC

Confirmation - LEAP-Seq

LEAP-Seq distance:1547
LEAP-Seq percent confirming:100.0
LEAP-Seq n confirming:3
LEAP-Seq n nonconfirming:0
LEAP-Seq n unique pos:3

Suggested primers for confirmation by PCR

Suggested primer 1:CAAGCAAACAGTGTCCCGTG
Suggested primer 2:TGTCACGTGTCTCTGAGCAC