Insertion junction: CLIP2.003599_1

Overview

Insertion cassette:CIB2
Side of cassette:3' truncated?
Strand:-
Strain:CLIP2.003599
Chromosome:chromosome 17
Location:3532716
Confidence (%):80
Locus systematic id Locus common name Defline Feature
Cre17.g724600 PAO2,TIC55-2 Pheophorbide a oxygenase-related protein; (1 of 3) K13071 - pheophorbide a oxygenase (PAO, ACD1) intron

Insertion site details

Expected flanking sequence (starting at cassette; orientation from cassette outwards): TTGGGAGTCGCTGTCCACGTTGGTGGTGTCCGTTTCCCCCTGCCGCTTGC
Internal bar code:AAGGGCGAGCTGTTCTTATGCA

Confirmation - LEAP-Seq

LEAP-Seq distance:1689
LEAP-Seq percent confirming:100.0
LEAP-Seq n confirming:17
LEAP-Seq n nonconfirming:0
LEAP-Seq n unique pos:17

Suggested primers for confirmation by PCR

Suggested primer 1:ATCCAGTGTTGCCCCTGATG
Suggested primer 2:CAGGCGTGGTTTTGGAGTTG