Insertion junction: CLIP2.003633_1

Overview

Insertion cassette:CIB2
Side of cassette:3'
Strand:-
Strain:CLIP2.003633
Chromosome:chromosome 12
Location:2017942
Confidence (%):80
Locus systematic id Locus common name Defline Feature
Cre12.g510650 FBP2,FBP1 Fructose-1%252C6-bisphosphatase, chloroplastic; (1 of 1) K03841 - fructose-1,6-bisphosphatase I (FBP, fbp) 3'UTR

Insertion site details

Expected flanking sequence (starting at cassette; orientation from cassette outwards): GAACCAATTGGCGGCGACTCAGGTAGACGGCGTCCTTTGAACGCGCGCTG
Internal bar code:AGTTGCAGTTCGTCTTAGACCA

Confirmation - LEAP-Seq

LEAP-Seq distance:3098
LEAP-Seq percent confirming:100.0
LEAP-Seq n confirming:22
LEAP-Seq n nonconfirming:0
LEAP-Seq n unique pos:22

Suggested primers for confirmation by PCR

Suggested primer 1:AGCGAGTGATTGGGAGTGTG
Suggested primer 2:CGACGCCAAGAACAAGAACG