Insertion junction: CLIP2.003644_1

Overview

Insertion cassette:CIB2
Side of cassette:3'
Strand:-
Strain:CLIP2.003644
Chromosome:chromosome 6
Location:8455062
Confidence (%):80
Locus systematic id Locus common name Defline Feature
Cre06.g308600 intron

Insertion site details

Expected flanking sequence (starting at cassette; orientation from cassette outwards): CCCCCTTATCACCGCACCCACAGGTCGCCATCCAGAAGCTGTTTCAGACA
Internal bar code:TAGTTTTGTGCTTCGTGGGGTC

Confirmation - LEAP-Seq

LEAP-Seq distance:2975
LEAP-Seq percent confirming:100.0
LEAP-Seq n confirming:116
LEAP-Seq n nonconfirming:0
LEAP-Seq n unique pos:116

Suggested primers for confirmation by PCR

Suggested primer 1:CTCCGTCTCCGTCTTCCATG
Suggested primer 2:AGCGTTGTGAAAGTTGTCGC