Insertion junction: CLIP2.003647_1

Overview

Insertion cassette:CIB2
Side of cassette:5'
Strand:-
Strain:CLIP2.003647
Chromosome:chromosome 1
Location:5886782
Confidence (%):80
Locus systematic id Locus common name Defline Feature
Cre01.g800103 (1 of 1) PF09749 - Uncharacterised conserved protein (HVSL) intron

Insertion site details

Expected flanking sequence (starting at cassette; orientation from cassette outwards): AAGTGCCACCGTTTGTCATGGTCATCATCCATGTTCGCTGAGTCCTGCAC
Internal bar code:CATGCCAATCGTGGTATGCGAG

Confirmation - LEAP-Seq

LEAP-Seq distance:1109
LEAP-Seq percent confirming:81.8182
LEAP-Seq n confirming:18
LEAP-Seq n nonconfirming:4
LEAP-Seq n unique pos:22

Suggested primers for confirmation by PCR

Suggested primer 1:AGCCTGGATCTGGATCTGGT
Suggested primer 2:CCCCATCTCCAACATGTGCT