| Insertion cassette: | CIB2 |
| Side of cassette: | 5' |
| Strand: | + |
| Strain: | CLIP2.003651 |
| Chromosome: | chromosome 16 |
| Location: | 7715970 |
| Confidence (%): | 80 |
| Locus systematic id | Locus common name | Defline | Feature |
|---|---|---|---|
| Cre16.g686510 | 3'UTR |
Insertion site details | |
| Expected flanking sequence (starting at cassette; orientation from cassette outwards): | TGTAGGACACTGTGGGAGGGGCCGGGGCAACGTGCGTGCCGGTTGGGGAG |
| Internal bar code: | ACTGTGAGAACGTAAGTCCAAG |
Confirmation - LEAP-Seq | |
| LEAP-Seq distance: | 2884 |
| LEAP-Seq percent confirming: | 67.6056 |
| LEAP-Seq n confirming: | 48 |
| LEAP-Seq n nonconfirming: | 23 |
| LEAP-Seq n unique pos: | 71 |
Suggested primers for confirmation by PCR | |
| Suggested primer 1: | TGGATCAGGAGTGGAGAGGG |
| Suggested primer 2: | AGGGGCCATGCATGTTTACA |