Insertion junction: CLIP2.003664_1

Overview

Insertion cassette:CIB2
Side of cassette:5'
Strand:-
Strain:CLIP2.003664
Chromosome:chromosome 1
Location:2593805
Confidence (%):80
Locus systematic id Locus common name Defline Feature
Cre01.g015350 POR1,POR Light-dependent protochlorophyllide reductase; (1 of 1) K00218 - protochlorophyllide reductase (E1.3.1.33, por) CDS

Insertion site details

Expected flanking sequence (starting at cassette; orientation from cassette outwards): TGCACGAACTGGCGCACCGACTCCAGGGAGGACAGGTCCAGGTGCAGGAT
Internal bar code:GGTTGATTTAGAGGGGGTGACT

Confirmation - LEAP-Seq

LEAP-Seq distance:2394
LEAP-Seq percent confirming:100.0
LEAP-Seq n confirming:9
LEAP-Seq n nonconfirming:0
LEAP-Seq n unique pos:9

Suggested primers for confirmation by PCR

Suggested primer 1:TACGGATCTTCGCTGTTCGG
Suggested primer 2:CCAAGTGCACGTGGTCAAAG