Insertion junction: CLIP2.003670_1

Overview

Insertion cassette:CIB2
Side of cassette:5'
Strand:-
Strain:CLIP2.003670
Chromosome:chromosome 2
Location:5003347
Confidence (%):80
Locus systematic id Locus common name Defline Feature
Cre02.g110750 LHCB7,LHCQ Light-harvesting chlorophyll a-b binding protein, chloroplast precursor; (1 of 1) K14172 - light-harvesting complex II chlorophyll a/b binding protein 7 (LHCB7) CDS

Insertion site details

Expected flanking sequence (starting at cassette; orientation from cassette outwards): TGGCACCGACTGTATTTATCCCTGCACACCCGCACGACCGCGCTGCCAGC
Internal bar code:TGTACCAGTTGATTCAGGCATG

Confirmation - LEAP-Seq

LEAP-Seq distance:5920
LEAP-Seq percent confirming:100.0
LEAP-Seq n confirming:112
LEAP-Seq n nonconfirming:0
LEAP-Seq n unique pos:112

Suggested primers for confirmation by PCR

Suggested primer 1:ACAGGAATGCTACCTTGGCC
Suggested primer 2:TCTGAGCAATGGTTCCCCAC