Insertion junction: CLIP2.003688_1

Overview

Insertion cassette:CIB2
Side of cassette:3'
Strand:+
Strain:CLIP2.003688
Chromosome:chromosome 13
Location:1407587
Confidence (%):80
Locus systematic id Locus common name Defline Feature
Cre13.g571950 (1 of 1) 2.1.1.104 - Caffeoyl-CoA O-methyltransferase / Trans-caffeoyl-CoA 3-O-methyltransferase CDS

Insertion site details

Expected flanking sequence (starting at cassette; orientation from cassette outwards): CACGGGTCGCACATGCAGGTAAGAGGGCAAGGGCACCTGAGAGGCGCGTA
Internal bar code:ATAACTTTACGGTCTAGGACCG

Confirmation - LEAP-Seq

LEAP-Seq distance:3833
LEAP-Seq percent confirming:100.0
LEAP-Seq n confirming:75
LEAP-Seq n nonconfirming:0
LEAP-Seq n unique pos:75

Suggested primers for confirmation by PCR

Suggested primer 1:CTCGGTTTCTCTGGGTTCCC
Suggested primer 2:GACACTGGTGACCCTGTCTG