Insertion junction: CLIP2.003699_1

Overview

Insertion cassette:CIB2
Side of cassette:3' truncated?
Strand:-
Strain:CLIP2.003699
Chromosome:chromosome 7
Location:2193420
Confidence (%):80
Locus systematic id Locus common name Defline Feature
Cre07.g327100 (1 of 12) IPR000326 - Phosphatidic acid phosphatase type 2/haloperoxidase CDS

Insertion site details

Expected flanking sequence (starting at cassette; orientation from cassette outwards): GAAGGGCAGCAGCAGGAGCAGGGGGAGCAGGGGCAGGGGCAGGGACAACG
Internal bar code:GTGTCCGCATGGCAACTGATAG

Confirmation - LEAP-Seq

LEAP-Seq distance:1311
LEAP-Seq percent confirming:82.8571
LEAP-Seq n confirming:29
LEAP-Seq n nonconfirming:6
LEAP-Seq n unique pos:35

Suggested primers for confirmation by PCR

Suggested primer 1:ATGGGGAGCAGGTTGGAATG
Suggested primer 2:GTCAAACTGGGGAGTGGAGG