Insertion junction: CLIP2.003703_1

Overview

Insertion cassette:CIB2
Side of cassette:3'
Strand:-
Strain:CLIP2.003703
Chromosome:chromosome 13
Location:3021798
Confidence (%):80
Locus systematic id Locus common name Defline Feature
Cre13.g584250 intron

Insertion site details

Expected flanking sequence (starting at cassette; orientation from cassette outwards): CATAGCTGGCCTAACCGCTGCGGCACGGGCCACGGAAACACGCCTTGTGC
Internal bar code:CTCTGAATTTTTGGTTTGCAAT

Confirmation - LEAP-Seq

LEAP-Seq distance:3680
LEAP-Seq percent confirming:99.2308
LEAP-Seq n confirming:129
LEAP-Seq n nonconfirming:1
LEAP-Seq n unique pos:130

Suggested primers for confirmation by PCR

Suggested primer 1:CGCTTTTCACGCTCCAACAA
Suggested primer 2:CTGCTGGGTGGCTTTTTGTC